NK Cell Activity Test: Cytotoxicity Analysis
Know Your Immune Health | Know Your Cancer Risk
We recommend every patient to perform an Immune System Test & Cancer Risk Test (called NK Activity Test or NK Cytotoxicity test). Natural Killer cell cytotoxic activity is vital for the clearance of viral (infected cells), scenecent cells and malignantly transformed cells (cancer cells). Our specialist protocols are used to assess NK cell cytotoxic activity against tumour cells, lytic proteins, degranulation and interferon gamma production.
Natural Killer (NK) cells are innate immune cells which lyse infected and malignantly transformed cells (cancer cells) by cytotoxic activity. Cytotoxic NK cells contain high numbers of secretory granules which store and release death inducing proteins such as perforin and granzymes. Perforin facilitates the delivery of serine proteases known as granzymes into the target cell by forming a pore either on the endosome or plasma membrane of the target cell. The granzymes released activate three distinct pathways of apoptosis in the target cell. In humans, granzyme B (GrzB) and granzyme A (GrzA) are the most potent activators of apoptosis (Cancer/infected cell death). NK cells also initiate target cell apoptosis (cancer/infected cell death) through tumour necrosis factor (TNF) ligands expressed on NK cells to induce apoptosis. ImmuneCells21 medical center has developed a pioneering weak immune system test to fight cancer and other autoimmune diseases.
Cancer Risk Test
NK Activity Test & Cytotoxicity Analysis
From your own blood we will extract your NK cells to test activity levels. The ability of NK cells to lyse tumour cells is measured by placing your NK cells with target cancer cells in a culture dish. The cells are then incubated together for 4 hours at 37°C with 5% CO2.
Post incubation, 7 amino-actinomycin D and fluorescein isothiocyanate are added to determine the number of cancer cells in apoptosis on flow cytometer. A total of 10,000 events are analysed and the number of events in each region are used to determine NK cell cytotoxic activity (number of successful deaths of cancer cells).
Natural Killer Cell Activity: Understanding Your Results
The average reference ranges for healthy patients are:
- Male: 43 – 58 %
- Female: 35 – 51 %
If you are within the reference range, indicating you have a healthy immune system we would recommend for you to cryo-store your immune cells to be used at a time in the future when they may be needed.
If your result is lower than the reference value, indicating you have an unhealthy immune system, this puts you in the higher risk category of developing cancer and infections. We would recommend to culture and activate your NK cells with our innovative weak immune system test.
Possible Reasons for Low Functioning Immune System:
A low functioning immune system puts risk to your health. Some possible reasons are:
- People who have cancer or has relatives known to have had cancer.
- Life style issues such as smoking, sleeplessness (less than 7 hours per day), stress, obesity.
- Poor nutrition such as lack of fruit and vegetables or proteins.
- People who take medical drugs such as immuno-suppressants and chemotherapy.
- People who take recreational drugs.
- Patients after surgery/anesthesias.
- Patients with Perforin deficiency, NEMO mutation.
People Strongly Advised to Have “NK Activity Testing“:
- People who have a family history or relatives with cancer.
- People who are frequently infected which reflect immune deficiency.
- People who have chronic diseases such as liver and kidney diseases, diabetes, and hyperlipidemia.
- People who have chronic hepatitis virus infection.
- People with ongoing cancer treatment, or who have completed cancer treatment.
- People who have little time for rest, a busy life styles, poor health issues, drink alcohol and smoke.
Other Areas to Check:
Tumor markers: CA19-9, colon, pancreas, liver, stomach. AFP, liver, stomach, pancreas, testes. CEA, stomach, colon, pancreas, lung and liver. HCG ovary, liver, lungs, stomach, rectum. HGH lung. CA 153 breast, egg, liver, lungs, stomach, rectum. f-PSA, prostate, endometrium, liver, lung, pancreas, breast. PSA, prostate, endometrium, liver, lung, pancreas, breast. NSE lung, pancreas, thyroid. CA 125, ovarian, liver, stomach, pancreas, breast, uterus. Ferritin, liver, breast, lung, pancreas.
Complete blood count (CBC), the integrity of the immune: Helper T cells, B cells, Suppressor T cells, Immunoglobulin level (IgM,IgG,IgA,IgE).
Check related substances: Vitamin A, Vitamin B12, Vitamin C, Vitamin D, Vitamin E, Folic acid, Zinc, Selenium, Copper.